Table 1 Multiplex PCR reverse cross blot hybridization results
From: Detection and identification of mycobacteria in sputum from suspected tuberculosis patients
PCR Probe | Mycobacterium Species | Â | Cultures | |
|---|---|---|---|---|
| Â | Â | Â | (+)ve | (-)ve |
pMyc5a | Mycobacterium spp | 5'-GGGCCCATCCCACACCGC-3' | 117 | 162 |
pAvi7 | M. avium | 5'CCAGAAGACATGCGTCTTGAG-3' | 3 | 5 |
pInt5 | M. intracellulare | 5'-CACCTAAAGACATGCGCCTAA-3' | 1 | 1 |
pInt7 | M. intracellulare | 5'-CACCAAAAGACATGCGTCTAA-3' | 1 | 1 |
pKan7 | M. kansasii | 5'CAAGGCATGCGCCAAGTGGT-3' | 1 | 1 |
pXen1 | M. xenopi | 5'-ACCACCCCACATGCGGAGAA-3' | 0 | 0 |
pFor1 | M. fortuitum | 5'-ACCACACACCATGAAGCGCG-3' | 1 | 1 |
pChe3 | M. chelonae | 5'-CCACTCACCATGAAGTGTGTG-3' | 2 | 3 |
pGen1 | M. genavense | 5'-CCACAAAACATGCGTTCCGTG-3' | 0 | 1 |
pGor5 | M. gordonae | 5'-TGTGTCCTGTGGTCCTATTCG-3 | 0 | 0 |
pMar2 | M. marinum | 5'-CGGGATTCATGTCCTGTGGT-3' | 0 | 0 |
Pt3 | M. tuberculosis complex | 5'-GAACGGCTGATGACCAAACT-3' | 117 | 162 |
pSme3 | M. smegmatis | 5'-CATGCGACCAGCAGGGTGTA-3' | 1 | 1 |
pTub1 | M. tuberculosis complex | 5'-AACACAAGACATGCATCCCG-3' | 108 | 149 |