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Figure 2 | BMC Research Notes

Figure 2

From: Opening the archives for state of the art tumour genetic research: sample processing for array-CGH using decalcified, formalin-fixed, paraffin-embedded tissue-derived DNA samples

Figure 2

Array-CGH plots of decalcified FFPE samples after ULS or RP labelling. For all plots: Upper right: Correlation plots of log2 ratios for each reporter between experiments, with linear regression and Pearson's correlation coefficients given. Lower left: Bland-Altman plots of the differences between two reporters measured by two experiments on the y axis against the mean log2 ratio of the two on the x axis. A: Correlation plots of sample Nr 10 comparing hybridisation of random prime and ULS based labelling of FFPE and RP labelling of frozen tissue derived DNA samples. B: Correlation plots of sample Nr 13 using FFPE isolated DNA samples with ULS, 150 ng RP and 500 ng RP labelling. C, D Correlation plots of samples Nr 2 and Nr 17 using FFPE isolated DNA samples with ULS or RP labelling reactions. E: Correlation plot of sample Nr 18. This sample showed a great degree of discrepancy for the estimated DNA concentration between the absorption based and the gel based measurements.

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