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Table 2 GeNorm ranking of the reference genes stability in all investigated tissues,.

From: Validation of reference genes for quantitative RT-qPCR studies of gene expression in Atlantic cod (Gadus morhua l.) during temperature stress

 

Best gene

2 nd best

3 rd best

4 th best

 

Stress

Control

Stress

Control

Stress

Control

Stress

Control

Liver

rplp1/ef1a

rplp1/ubiq

18s

tba1c

g6pd

g6pd

actb

actb

Heart

ubiq/ef1a

ubiq/18s

18s

rplp1

rplp1

actb

actb

ef1a

Intestine

actb/ubiq

g6pd/18s

ef1a

rplp1

rplp1

ef1a

tba1c

actb

Spleen

rplp1/ubiq

actb/ubiq

18s

tba1c

ef1a

rplp1

actb

18s

Kidney

ubiq/ef1a

rplp1/ef1a

rplp1

g6pd

b2m

tba1c

18s

ubiq

Gill

g6pd/actb

tba1c/ubiq

ubiq

rplp1

tba1c

ef1a

18s

actb

Pooled tissues

ubiq/rplp1

ubiq/18s

b2m

ef1a

18s

rplp1

ef1a

tba1c

  1. Stability ranking of individual reference genes in different tissues from Atlantic cod under control (stable temperature) or stress situation (temperature increase of 1?C/day for 5 days) conditions.