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Table 4 Bestkeeper analysis of reference genes from Atlantic cod.

From: Validation of reference genes for quantitative RT-qPCR studies of gene expression in Atlantic cod (Gadus morhua l.) during temperature stress

Pearson correlation coefficient (r)        
  rplp1 g6pd tba1c actb b2m ubiq ef1a 18s
vs. HKG 1 HKG 2 HKG 3 HKG 4 HKG 5 HKG 6 HKG 7 HKG 8
g6pd 0,981 - - - - - - -
p-value 0,001 - - - - - - -
tba1c 0,685 0,688 - - - - - -
p-value 0,029 0,028 - - - - - -
actb 0,865 0,885 0,853 - - - - -
p-value 0,001 0,001 0,002 - - - - -
b2m 0,012 0,081 0,284 0,390 - - - -
p-value 0,977 0,824 0,425 0,264 - - - -
ubiq 0,857 0,800 0,765 0,868 0,297 - - -
p-value 0,002 0,005 0,010 0,001 0,405 - - -
ef1a 0,922 0,929 0,592 0,754 -0,147 0,707 - -
p-value 0,001 0,001 0,071 0,012 0,686 0,022 - -
18s 0,537 0,516 0,647 0,600 0,276 0,676 0,573 -
p-value 0,110 0,128 0,043 0,067 0,441 0,032 0,083 -
RANK 3 2 6 1 8 4 5 7
BestKeeper vs. rplp1 g6pd tba1c actb b2m ubiq ef1a 18s
coeff. of corr. [r] 0,950 0,954 0,816 0,960 0,265 0,912 0,871 0,683
p-value 0,001 0,001 0,004 0,001 0,458 0,001 0,001 0,030
  1. Bestkeeper analysis of candidate reference genes from various tissues in Atlantic cod. Pair wise correlation analysis showing Pearson correlation coefficient (r) and the value of probability, p. Pair of genes with r > 0,80 in bold.