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Table 4 Bestkeeper analysis of reference genes from Atlantic cod.

From: Validation of reference genes for quantitative RT-qPCR studies of gene expression in Atlantic cod (Gadus morhua l.) during temperature stress

Pearson correlation coefficient (r)

       
 

rplp1

g6pd

tba1c

actb

b2m

ubiq

ef1a

18s

vs.

HKG 1

HKG 2

HKG 3

HKG 4

HKG 5

HKG 6

HKG 7

HKG 8

g6pd

0,981

-

-

-

-

-

-

-

p-value

0,001

-

-

-

-

-

-

-

tba1c

0,685

0,688

-

-

-

-

-

-

p-value

0,029

0,028

-

-

-

-

-

-

actb

0,865

0,885

0,853

-

-

-

-

-

p-value

0,001

0,001

0,002

-

-

-

-

-

b2m

0,012

0,081

0,284

0,390

-

-

-

-

p-value

0,977

0,824

0,425

0,264

-

-

-

-

ubiq

0,857

0,800

0,765

0,868

0,297

-

-

-

p-value

0,002

0,005

0,010

0,001

0,405

-

-

-

ef1a

0,922

0,929

0,592

0,754

-0,147

0,707

-

-

p-value

0,001

0,001

0,071

0,012

0,686

0,022

-

-

18s

0,537

0,516

0,647

0,600

0,276

0,676

0,573

-

p-value

0,110

0,128

0,043

0,067

0,441

0,032

0,083

-

RANK

3

2

6

1

8

4

5

7

BestKeeper vs.

rplp1

g6pd

tba1c

actb

b2m

ubiq

ef1a

18s

coeff. of corr. [r]

0,950

0,954

0,816

0,960

0,265

0,912

0,871

0,683

p-value

0,001

0,001

0,004

0,001

0,458

0,001

0,001

0,030

  1. Bestkeeper analysis of candidate reference genes from various tissues in Atlantic cod. Pair wise correlation analysis showing Pearson correlation coefficient (r) and the value of probability, p. Pair of genes with r > 0,80 in bold.