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Table 1 Primers used for detection

From: Occurrence of genes of putative fibrinogen binding proteins and hemolysins, as well as of their phenotypic correlates in isolates of S. lugdunensis of different origins

Gene/locus-tag

Name

Sequence 5' 3'

Size (bp)

Primer

fbl

fbl_check_F

CGTATTATCCCAAGTAGCAACC

404

This study

 

fbl_check_R

CTTCATCGATTGTCCCAGTAGC

  

SLGD_01696

FbpA_F

GAGATTACTGGACAACAAACG

558

This study

 

FbpA_R

GTATTGTGACGTCGTTTCCTG

  

SLGD_00006

betahemolysin_F

TGGTCAAGGTACAGAAGGTTGGCA

449

This study

 

betahemolysin_R

TATCCCAACTATACGCGTTGCCCT

  

SLGD_00847

hemolysinIII_F

TAATGCTGTTTCGCACGGAGTTGC

407

This study

 

hemolysinIII_R

GACGCCTACCCATCCCATTACAA

  

SLUSH-cluster

slush_donvito_F

TTTCGTCTTTGCACACACATTTCCA

977

This study

 

slush_donvito_R

ACAGCACAAAGCCTTAACTATCTCA

  

SLGD_02429

stlu_vwbl_F

TGGCGGGATGATTTGGACGGG

858

This study

vwbl

stlu_vwbl_R

TCGCCTTCTTGCCCTGATGGT

  
  1. The previously published fibrinogen binding protein gene (fbl) sequences [8, 9], the von Willebrand factor binding protein precursor gene (vwbl) sequences (AY530288) [18] and SLGD_02429 [13], the putative fibrinogen/fibronectin binding protein (FbpA homologue SLGD_01696) gene sequence [13], the S. lugdunensis synergistic hemolysin (SLUSH) gene sequence (U73444.1) [11], the S. lugdunensis putative beta-hemolysin (SLGD_00006) gene sequence [11] and the S. lugdunensis putative hemolysin III (SLGD_00847) gene sequence [11] were used to design primer pairs (This study).