|
Gene/locus-tag
|
Name
|
Sequence 5' 3'
|
Size (bp)
|
Primer
|
|---|
|
fbl
|
fbl_check_F
|
CGTATTATCCCAAGTAGCAACC
|
404
|
This study
|
| |
fbl_check_R
|
CTTCATCGATTGTCCCAGTAGC
| | |
|
SLGD_01696
|
FbpA_F
|
GAGATTACTGGACAACAAACG
|
558
|
This study
|
| |
FbpA_R
|
GTATTGTGACGTCGTTTCCTG
| | |
|
SLGD_00006
|
betahemolysin_F
|
TGGTCAAGGTACAGAAGGTTGGCA
|
449
|
This study
|
| |
betahemolysin_R
|
TATCCCAACTATACGCGTTGCCCT
| | |
|
SLGD_00847
|
hemolysinIII_F
|
TAATGCTGTTTCGCACGGAGTTGC
|
407
|
This study
|
| |
hemolysinIII_R
|
GACGCCTACCCATCCCATTACAA
| | |
|
SLUSH-cluster
|
slush_donvito_F
|
TTTCGTCTTTGCACACACATTTCCA
|
977
|
This study
|
| |
slush_donvito_R
|
ACAGCACAAAGCCTTAACTATCTCA
| | |
|
SLGD_02429
|
stlu_vwbl_F
|
TGGCGGGATGATTTGGACGGG
|
858
|
This study
|
|
vwbl
|
stlu_vwbl_R
|
TCGCCTTCTTGCCCTGATGGT
| | |
- The previously published fibrinogen binding protein gene (fbl) sequences [8, 9], the von Willebrand factor binding protein precursor gene (vwbl) sequences (AY530288) [18] and SLGD_02429 [13], the putative fibrinogen/fibronectin binding protein (FbpA homologue SLGD_01696) gene sequence [13], the S. lugdunensis synergistic hemolysin (SLUSH) gene sequence (U73444.1) [11], the S. lugdunensis putative beta-hemolysin (SLGD_00006) gene sequence [11] and the S. lugdunensis putative hemolysin III (SLGD_00847) gene sequence [11] were used to design primer pairs (This study).
