Tissue autofluorescence. a - d) These figures show four non treated (native) CNV membranes at20 × magnification. a) Shows bright field and Fig 3b-d the autofluorescence of lipofuscin in red, green and blue field. Although different wave length were used, tissue autofluorescence is present at various wavelengths so that nucleus staining (DAPI) and Hsp70-detection could not be performed. Arrow indicates the retinal pigment epithelium (RPE).