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Table 2 Patient's demographic data and fingerprinting data for the four individual patient multidrug-resistant M. tuberculosi s strains containing a mutation in quinolone resistance-determining region (QRDR) of gyrA gene.

From: First report of molecular detection of fluoroquinolone resistance-associated gyrA mutations in multidrug-resistant clinical Mycobacterium tuberculosis isolates in Kuwait

Patient Nationality Clinical Year of Resistance Mutation in QRDR Fingerprinting data from analysis ofb
no.   specimen isolation patterna of gyrA gene rpoB c inhA -RR d Genetic groupe
1 Indian Pleural fluid 2004 H, R, S, E GAC94GGC/D94G Wild-typef WT I
2 Bangladeshi Sputum 2006 H, R, S, E GAC94GCC/D94A H526Q + D516G WT III
3* Indian Sputum 2006 H, R, E GAC94GGC/D94G I572F WT I
4 Indian Sputum 2008 H, R, E GAC94GGC/D94G S531L -17G→T I
  1. aH, isoniazid; R, rifampicin, S, streptomycin; E, ethambutol
  2. bAll four were male patients and all individual patient isolates contained AGC315ACC mutation in katG codon 315 and yielded unique patterns in DRE-PCR
  3. cThree (hot-spot, N-terminal and cluster II) regions of rpoB gene were sequenced to detect rifampicin resistance-conferring mutations and codon numbering system of rpoB gene from Escherichia coli is used [4]
  4. dInhA-RR, inhA regulatory region sequencing data
  5. eGenetic groups were assigned based on polymorphisms at katG codon 463 and gyrA codon 95(L463/T95, Group I; R463/S95, Group III)
  6. fThis isolate either contained a mutation in other regions of the rpoB gene or in other genes involved in rifampicin resistance [25]
  7. *Two repeat isolates recovered from this patient exhibited identical patterns