Figure 2
In vitro proteolytic degradation of invasin. Y. enterocolitica WA-314 (wt) or the invasin mutant (inv) were digested for 2 h at 37°C with small intestinal fluid of mice (SI) in different dilutions (1:10 - 1:10000). Digests with trypsin and trypsin digestion buffer (control) are also shown. The small intestines of mice were washed with 2 ml trypsin digestion buffer, centrifuged, and the supernatant was used to digests 3 × 106 CFU of yersiniae. Immunoblotting was performed with an a inv polyclonal antibody. Solid arrowheads point to full length Inv, open arrowheads point to specific Inv degradation products.