Figure 3
Kinetics of proteolysis by human ileal fluid. 108 CFU Y. enterocolitica WA-314 (wt) or the invasin mutant (inv) were digested at 37°C for the indicated time with 1 ml human ileal fluid. For each lane 3 × 106 yersiniae were subjected to Westerm blotting with an a Inv polyclonal AB. Solid arrowheads point to full length Inv, open arrowheads point to specific Inv degradation products.