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Table 1 Results of applying the Phred quality filter

From: Analysis of quality raw data of second generation sequencers with Quality Assessment Software

 

Raw data

Filter by Mean

Filter by Median

Cp162

21,102,241

11,349,208(53.78%)

13,140,825(62.27%)

B7(F3)

44,171,676

24,927,365(56.46%)

28,216,025(63.87%)

B7(R3)

45,024,226

31,809,614(70.64%)

33,765,330 (74.99%)

  1. Cp162- Corynebacterium pseudotuberculosis strain (fragment library).
  2. B7 - Exiguobacterium antarticum strain (mate-paired library).
  3. R3 e F3 - Reads generated by mate-paired library.
  4. Number of reads generated during the sequencing of Cp162 and B7 (tag F3 and R3) taking into account both the raw data and filtered data, based on the average and median Phred quality, whose cutoff was taken to be 20.