Skip to main content

Table 2 PCR conditions.

From: Expression of multiple forms of 3'-end variant CCK2 receptor mRNAs in human pancreatic adenocarcinomas

PCR No. Initial denaturation Denaturation Annealing Extension Cycles Final extension
1 95°C, 15 min 95°C, 30 s 65°C, 30 s 72°C, 1 min 35 72°C, 10 min
2 95°C, 15 min 95°C, 30 s 65°C, 30 s 72°C, 1 min 30 72°C, 10 min
3 95°C, 15 min 95°C, 30 s 55°C, 30 s 72°C, 1 min 30 72°C, 10 min
4 95°C, 15 min 95°C, 30 s 52°C, 30 s 72°C, 1 min 30 72°C, 10 min