Figure 1

Cell viability and transfection efficiency after nucleofection of TO cells. A total of 5 × 10⁶ cells were transfected with 5 μg pFRT/lacZeo Flp, using buffer R, T or V, and a range of programs. Three days after transfection, cell viability (a) and GFP expression (b) was quantified by flow cytometry. * = Electroporation, no plasmid ** = Plasmid, no electroporation program. Data from a representative experiment (out of 3) is depicted here.