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Table 1 Sequences of variants exhibiting β-galactosidase activity.

From: Applying neutral drift to the directed molecular evolution of a β-glucuronidase into a β-galactosidase: Two different evolutionary pathways lead to the same variant

 

Wild Type

A

4.1

A

4.3

A

4.5

A

5.1

A

5.2

B

4.1

C

4.1

C

4.2

Neutral 1

Neutral 2

Shuffled-1

Shuffled-2

AA substitutions

 

2

3

4

4

3

2

3

3

2

5

  

Silent Mutations

 

2

1

1

3

0

1

      

Position

             

36

E

         

K

  

42

S

          

N

 

57

D

   

N

        

67

V

       

D

    

81

G

    

V

      

V

185

D

         

G

  

168

A

    

S

       

217

R

  

C

         

245

G

      

A

     

266

P

         

S

  

278

G

 

A

          

308

N

 

D

          

313

H

         

Y

  

367

A

  

P

         

370

K

      

R

R

    

378

E

          

R

 

480

T

     

A

      

484

V

        

I

   

498

Q

           

R

508

D

   

G

        

509

T

 

A

A

       

A

A

510

L

F

           

529

W

    

L

 

L

L

    

533

Y

   

C

        

536

V

        

I

   

540

V

  

D

         

557

S

   

A

      

P

P

566

N

S

    

S

    

S

S

568

K

          

Q

Q

594

G

         

D

  
  1. Variants A4.1 - 4.5 were obtained from experiment A after four rounds of neutral screening. Variants A5.1 and A5.2 were obtained from the fifth round of neutral screening. Variant B4.1 was obtained from experiment B using a high mutagenesis rate, after four rounds of neutral screening. Variants C4.1 and C4.2 were obtained from experiment C after four rounds of neutral screening. Shuffled-1 and Shuffled-2 are the variants obtained by DNA shuffling of the variants with galactosidase activity. The positions where mutations were previously identified by Matsumura and Ellington [13] are highlighted.
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BMC Research Notes

ISSN: 1756-0500

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