Figure 3
From: Analysis of the anti-tumor effect of cetuximab using protein kinetics and mouse xenograft models
Kinetics of proteins involved in EGFR/MAPK signaling with cetuximab in HT29 cells. A, HT29 cells were treated with serum-free RPMI for 24 hours, followed by the addition of EGF 100ng/ml, TGF-α 50ng/ml, IGF 50ng/ml, and cetuximab 300ng/ml, resulting in a total of 7 conditions for 0, 1, 5, 10, 15 minutes before collections of protein lysate. The phosphorylation of EGFR, MEK, and ERK was visualized using Western blot analysis. Actin was used as a loading control. B, A Western blot band was plotted in an arbitrary unit showing the time course.