Figure 1

SNPmplexViewer web form. A brief description of the program is followed by web links to the source code and to an example data set and its associated results. The input section contains the two input fields for uploading the reference and target traces and the checked box indicates that the defaults for the 25-plex traceability system should be used. The "200" option is selected in the radio buttons to signify that this was the optimal signal value for the normalisation procedure. Clicking the "align" button submits the form to the server which returns the records of the basic input parameters. The results section first indicates the sequencer identity and the position of the first signal peaks for each of the dyes and the actual scan at which the analysis started after trimming of the blank trace. The link "Download Modified Trace" allows downloading the normalised and modified target trace file in which the reference size standards are embedded. The upper and lower electropherograms are of the reference and target traces, respectively. These were well aligned as indicated by the difference trace between them.