Figure 2From: The importance of RT-qPCR primer design for the detection of siRNA-mediated mRNA silencingApparent knockdown of RRM1 mRNA measured by RT-qPCR following intratumoural injection of siRNA. A549 xenograft tumours were injected with PBS, RRM1-2, RRM1-3 or non-targeting siRNA (81c) (n = 5). RRM1 mRNA was measured by real-time RT-qPCR. PCR was carried out with primers flanking the RRM1-2 (RRM1-B, shown in (A)) or RRM1-3 site (RRM1-C, shown in (B)).Back to article page