Table 1 Primer and probe sequences used in OrfX-SCC PCR
primer/probe | Sequence 5'-3' | Reference |
|---|---|---|
F1 | GTCAAAAATCATGAACCTCATTACTTATG | according to Huletsky et al. |
F2 | AATATTTCATATATGTAATTCCTCCACATCTC | adapted from Huletsky et al. |
F3 | CTTCAAATATTATCTCGTAATTTACCTTGTTC | adapted from Huletsky et al. |
F4 | CTCTGCTTTATATTATAAAATTACGGCTG | according to Huletsky et al. |
F5 | TCACTTTTTATTCTTCAAAGATTTGAGC | adapted from Huletsky et al. |
F7 | CCATTTCTTCCAAAAAATATATTTACTTTAGTC | This study |
F8 | TTTCATAATATGTGCTACGCAACCTA | This study |
F9 | CGAGTTAATTTTTTATTTTAGAGCGCTTAC | This study |
F10 | CCGCTCCTTTTATATTATACACAACCTATT | This study |
F11 | GCCATATTAATGCCTCACGAAAC | This study |
F12 | CATTCATTAACATCGTACTCTGCATTT | This study |
F13 | TCCCTTTATGAAGCGGCTGAA | This study |
F14 | AAGCTATAGTTCAGCATTATCGTAAGTTAACT | This study |
F15 | TGCCAATCACAGTTCAATCAATTATT | This study |
F16 | TCCTTTCTAATTATATTATGCGCAACCT | This study |
F17 | ACTCTGATAAGCCATTCATTCATCCA | This study |
F18 | ACAATCCTAACATAAGATTGTGGCTTT | This study |
F20 | GCATATTCACTTTGATAAGCCATTCAT | This study |
F21 | CGGTTCTGATATCTTTTCAACCATT | This study |
F23 | CCCCTCCCATTAACTCCGTATAT | This study |
F24 | CCCAAACTCTTAACTTTCTTCAATACATT | This study |
F25 | TTCTAAGGTAGCTTCCCTTTCAATAATTT | This study |
R1 | CGTCATTGGCGGATCAAAC | adapted from Huletsky et al. |
R2 | CGTCATTGGTGGATCAAACG | adapted from Huletsky et al. |
probe2 | FAM-CACAAGGATGTCTTACAACG-MGB | adapted from Huletsky et al. |
probe3 | FAM-CACAAGGACGTCTTACAACG-MGB | adapted from Huletsky et al. |
probe4 | FAM-CACAAAGACGTCTTACAACG-MGB | adapted from Huletsky et al. |