Figure 2

Tumor lysates from cells which have been harvested using EDTA induce DC maturation in a dose dependent manner. HCT-116 colorectal tumor cells were harvested by using 0.02% EDTA to detach the cells, washed and lysed in PBS by repeated freezing and thawing. Human monocyte-derived immature DCs were stimulated for 3 days with supernatants of indicated concentrations of lysed tumor cells. Shown are representative results from 6 individual experiments with lysates from 2 different cell lines (HCT-116, CACO-2).