Tag name | Sequence | Tm (°C) |
|---|
F9GAC | 5' CTAGTATC AGGACGAC 3' | 51.3 |
F9GTC | 5' CTAGTATG AGGACGTC 3' | 51.3 |
F9TAC | 5' CTAGTATC AGGACTAC 3' | 47.2 |
F9GCC | 5' CTAGTATT AGGACGCC 3' | 51.7 |
F9CCG | 5' CTAGTATT AGGACCCG 3' | 50.8 |
F9AGG | 5' CTAGTATT AGGACAGG 3' | 47.5 |
- Nucleotide sequences of six BStags. One of these tag sequences was attached to the 5' end of a forward primer of a sequence-specific primer. The double underlined three nucleotides represent a 'bar-code' sequence. The single underlined nucleotide corresponds to the mismatched nucleotide in Figure 1A. Estimated melting temperatures (Tm) were obtained with PerlPrimer version 3[13].
