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Table 1 Description of candidate reference genes for qRT-PCR

From: Selection of reference genes for normalization of quantitative real-time PCR in organ culture of the rat and rabbit intervertebral disc

Abbreviation

Gene

Function

Actb

β-actin

Cytoskeletal structural protein

GAPDH

Glyceraldehydes-3-phosphate dehydrogenase

Carbohydrate metabolism

18S rRNA

18S ribosomal RNA

Cytosolic small ribosomal subunit, translation

CycA

Cyclophilin A

Catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides, accelerating folding

Hprt1

Hypoxanthine phosphoribosyltransferase 1

Metabolic salvage of purines in mammals

Ywhaz

Tyrosine 3-monooxygenase

Signal transduction by binding to phosphorilated serine residue on a variety of signaling molecules

Pgk1

Phosphoglycerate kinase 1

Transferase enzyme in the glycolysis