Selection of reference genes for normalization of quantitative real-time PCR in organ culture of the rat and rabbit intervertebral disc

Table 2 Primer information of reference genes for qRT-PCR

	Sprague-Dawley rat		New Zealand White Rabbit
	Forward (5'-3') Reverse (5'-3')	Product size (bp) [Ref]	Forward (5'-3') Reverse (5'-3')	Product size (bp) [Ref]
Actb	AGGCCAACCGTGAAAAGATG ACCAGAGGCATACAGGGACAA	101 [33]	CTGGAACGGTGAAGGTGACA CGGCCACATTGCAGAACTTT	73 [D]
GAPDH	GCAAGAGAGAGGCCCTCAG TGTGAGGGAGATGCTCAGTG	74 [18]	GGGTGGTGGACCTCATGGT CGGTGGTTTGAGGGCTCTTA	58 [D]
18S rRNA	ACGGACCAGAGCGAAAGCAT TGTCAATCCTGTCCGTGTCC	310 [19]	TCGGCATTCGAACGTATGC ACCCGTGGTCACCATGGTA	56 [D]
CycA	TATCTGCACTGCCAAGACTGAGTG CTTCTTGCTGGTCTTGCCATTCC	126 [20]	CCAACGGCTCCCAGTTCTT ACGTGCTTGCCGTCCAA	61 [D]
Hprt1	TGTTTGTGTCATCAGCGAAAGTG ATTCAACTTGCCGCTGTCTTTTA	66 [D]	GCAGACCTTGCTTTCCTTGGT GCAGGCTTGCGACCTTGAC	63 [D]
Ywhaz	TTGAGCAGAAGACGGAAGGT GAAGCATTGGGGATCAAGAA	136 [19]	GGTCTGGCCCTTAACTTCTCTGTGTTCTA GCGTGCTGTCTTTGTATGATTCTTCACTT	142 [28]
Pgk1	ATGCAAAGACTGGCCAAGCTAC AGCCACAGCCTCAGCATATTTC	104 [20]	TGTTGGTCGGGCGAAGCAG CAGTGTCTCCACCGCCGATG	149 [28]

ISSN: 1756-0500