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Table 3 Standard curve parameters for candidate reference genes

From: Selection of reference genes for normalization of quantitative real-time PCR in organ culture of the rat and rabbit intervertebral disc

  SD rat NZW rabbit
Genes Slope Efficiency (E) [%] Coefficient (R2) Slope Efficiency (E) Coefficient (R2)
Actb -3.024 2.141 [114.1] 0.992 -3.363 1.983 [98.3] 0.999
GAPDH -3.222 2.043 [104.3] 0.995 -3.388 1.973 [97.3] 0.997
18S rRNA -3.253 2.030 [103.0] 0.982 -3.584 1.901 [90.1] 0.994
CycA -3.135 2.084 [108.4] 0.996 -3.091 2.106 [110.6] 0.999
Hprt1 -3.360 1.984 [98.4] 0.991 -3.411 1.964 [96.4] 0.990
Ywhaz -3.043 2.131 [113.1] 0.994 -3.040 2.133 [113.3] 0.985
Pgk1 -3.078 2.113 [104.6] 0.992 -3.039 2.133 [113.3] 0.986