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Table 3 Standard curve parameters for candidate reference genes

From: Selection of reference genes for normalization of quantitative real-time PCR in organ culture of the rat and rabbit intervertebral disc

 

SD rat

NZW rabbit

Genes

Slope

Efficiency (E) [%]

Coefficient (R2)

Slope

Efficiency (E)

Coefficient (R2)

Actb

-3.024

2.141 [114.1]

0.992

-3.363

1.983 [98.3]

0.999

GAPDH

-3.222

2.043 [104.3]

0.995

-3.388

1.973 [97.3]

0.997

18S rRNA

-3.253

2.030 [103.0]

0.982

-3.584

1.901 [90.1]

0.994

CycA

-3.135

2.084 [108.4]

0.996

-3.091

2.106 [110.6]

0.999

Hprt1

-3.360

1.984 [98.4]

0.991

-3.411

1.964 [96.4]

0.990

Ywhaz

-3.043

2.131 [113.1]

0.994

-3.040

2.133 [113.3]

0.985

Pgk1

-3.078

2.113 [104.6]

0.992

-3.039

2.133 [113.3]

0.986