Figure 2From: Effect of different UCOE-promoter combinations in creation of engineered cell lines for the production of Factor VIIITransient transfection studies in adherent BHK21 cells comparing the different UCOE plasmids. Adherent BHK21 cells were transfected with equimolar amounts of the UCOE plasmids listed in figure 1 and the FVIII activity/ml (A), and the BDD protein levels secreted as measured by ELISA (B) were measured. Data was statistically analyzed using 't' test to detect significant differences between the 3.2 Kb and the 1.5 Kb UCOE containing expression constructs (*: p < 0.05 or more).Back to article page