Design of constructs, confirmation of transcription and translation of rice proteins in E. coli. a. Linear maps of the recombinant constructs employed in this study. b. RT-PCR analysis of IPTG un-induced and induced samples of transformed E. coli M15 cells. Upper panel shows RT-PCR depicting expression of the respective genes. Lower panel shows EtBr stained gel depicting comparable staining of the ribosomal RNA species, 2 μg of total RNA was loaded in each lane. c. Expression of the foreign proteins in the transformed E. coli cells. Total protein from the IPTG un-induced and induced cells were separately analyzed. Induced proteins are indicated by arrows. Pre-stained markers are shown on the left side of the panel. UI and I refer to IPTG un-induced and induced samples, respectively.