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Table 2 Strains and plasmids used in this study.

From: The luxS mutation causes loosely-bound biofilms in Shewanella oneidensis

Strain Description and genotype Sources/References
Shewanella oneidensis
MR-1 Wild type strain BCC/[2]
luxS - ins pKnock-Km plasmid inserted into the luxS gene this study
WTKm pKnock-Km plasmid inserted before the luxS gene this study
Escherichia coli
S17-1λpir Biparental mating
TpR SmR rec A thi pro hsd R RP4-2-Tc::Mu-Km::Tn7 λpir
Biomedal (Spain)/[29]
pir-116 Maintaining of R6K ori plasmids
F- mcr A Δ(mrr-hsd RMS-mcr BC) Φ80dlac ZΔM15 Δlac X74 rec A1 end A1 ara D139 Δ(ara, leu)7697 gal U gal K λ- rps L nup G pir-116(DHFR)
Epicentre/[30]
DH5α Subcloning routine
F- Φ80lac ZΔM15 Δ(lac ZYA-arg F)U169 rec Al end AI hsd R17(rk-, mk+) pho A sup E44 thi-1 gyr A96 rel AI λ-
Invitrogen/[31]
HB101 Carrier of the pRK2013 plasmid for triparental mating.
F- sup E44 lac Y1 ara-14 gal K2 xyl-5 mtl-1 leu B6 Δ(mcr C-mrr) rec A13 rps L20 thi-1 Δ(gpt-pro A)62 hsd SB20 λ-
[32]
Vibrio harveyi
BB152, BAA-1119 luxM::Tn5; produces only AI-2 ATCC/[33]
MM77 luxM::Tn5, luxS::CmR; produces neither AI-1 nor AI-2 ATTC/[34]
BB170, BAA-1117 luxN::Tn5; senses only AI-2 ATCC/[33]
Plasmids
pKnock-Km KmR; ori T; source plasmid for the suicide vectors into Sh. oneidensis NCCB 3407/[24]
pEX18Ap AmpR; source plasmid for a replicating plasmid into Sh. oneidensis Max Schobert, TU Braunschweig/[35]
pPS858 AmpR, GmR; source of the Gentamicin-GFP (Gm-GFP) cassette Max Schobert, TU Braunschweig/[35]
pRK2013 KmR; helper plasmid for conjugation DSMZ 5599/[36]
pKnock-luxS Construction of luxS-ins
KmR; pKnock-Km containing an internal fragment of luxS of Sh. oneidensis
This study
pBA2106 Construction of WTKm
KmR, pKnock-Km containing an upstream fragment of luxS of Sh. oneidensis
This study
pEX18ApGm For tagging Sh. oneidensis with gfp
KmR, GmR, the Gm-GFP cassette ligated into pEX18Ap
This study