From: The luxS mutation causes loosely-bound biofilms in Shewanella oneidensis
Strain | Description and genotype | Sources/References |
---|---|---|
Shewanella oneidensis | ||
MR-1 | Wild type strain | BCC/[2] |
luxS - ins | pKnock-Km plasmid inserted into the luxS gene | this study |
WTKm | pKnock-Km plasmid inserted before the luxS gene | this study |
Escherichia coli | ||
S17-1λpir | Biparental mating TpR SmR rec A thi pro hsd R RP4-2-Tc::Mu-Km::Tn7 λpir | Biomedal (Spain)/[29] |
pir-116 | Maintaining of R6K ori plasmids F- mcr A Δ(mrr-hsd RMS-mcr BC) Φ80dlac ZΔM15 Δlac X74 rec A1 end A1 ara D139 Δ(ara, leu)7697 gal U gal K λ- rps L nup G pir-116(DHFR) | Epicentre/[30] |
DH5α | Subcloning routine F- Φ80lac ZΔM15 Δ(lac ZYA-arg F)U169 rec Al end AI hsd R17(rk-, mk+) pho A sup E44 thi-1 gyr A96 rel AI λ- | Invitrogen/[31] |
HB101 | Carrier of the pRK2013 plasmid for triparental mating. F- sup E44 lac Y1 ara-14 gal K2 xyl-5 mtl-1 leu B6 Δ(mcr C-mrr) rec A13 rps L20 thi-1 Δ(gpt-pro A)62 hsd SB20 λ- | [32] |
Vibrio harveyi | ||
BB152, BAA-1119 | luxM::Tn5; produces only AI-2 | ATCC/[33] |
MM77 | luxM::Tn5, luxS::CmR; produces neither AI-1 nor AI-2 | ATTC/[34] |
BB170, BAA-1117 | luxN::Tn5; senses only AI-2 | ATCC/[33] |
Plasmids | ||
pKnock-Km | KmR; ori T; source plasmid for the suicide vectors into Sh. oneidensis | NCCB 3407/[24] |
pEX18Ap | AmpR; source plasmid for a replicating plasmid into Sh. oneidensis | Max Schobert, TU Braunschweig/[35] |
pPS858 | AmpR, GmR; source of the Gentamicin-GFP (Gm-GFP) cassette | Max Schobert, TU Braunschweig/[35] |
pRK2013 | KmR; helper plasmid for conjugation | DSMZ 5599/[36] |
pKnock-luxS | Construction of luxS-ins KmR; pKnock-Km containing an internal fragment of luxS of Sh. oneidensis | This study |
pBA2106 | Construction of WTKm KmR, pKnock-Km containing an upstream fragment of luxS of Sh. oneidensis | This study |
pEX18ApGm | For tagging Sh. oneidensis with gfp KmR, GmR, the Gm-GFP cassette ligated into pEX18Ap | This study |