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Table 2 Strains and plasmids used in this study.

From: The luxS mutation causes loosely-bound biofilms in Shewanella oneidensis

Strain

Description and genotype

Sources/References

Shewanella oneidensis

MR-1

Wild type strain

BCC/[2]

luxS - ins

pKnock-Km plasmid inserted into the luxS gene

this study

WTKm

pKnock-Km plasmid inserted before the luxS gene

this study

Escherichia coli

S17-1λpir

Biparental mating

TpR SmR rec A thi pro hsd R RP4-2-Tc::Mu-Km::Tn7 λpir

Biomedal (Spain)/[29]

pir-116

Maintaining of R6K ori plasmids

F- mcr A Δ(mrr-hsd RMS-mcr BC) Φ80dlac ZΔM15 Δlac X74 rec A1 end A1 ara D139 Δ(ara, leu)7697 gal U gal K λ- rps L nup G pir-116(DHFR)

Epicentre/[30]

DH5α

Subcloning routine

F- Φ80lac ZΔM15 Δ(lac ZYA-arg F)U169 rec Al end AI hsd R17(rk-, mk+) pho A sup E44 thi-1 gyr A96 rel AI λ-

Invitrogen/[31]

HB101

Carrier of the pRK2013 plasmid for triparental mating.

F- sup E44 lac Y1 ara-14 gal K2 xyl-5 mtl-1 leu B6 Δ(mcr C-mrr) rec A13 rps L20 thi-1 Δ(gpt-pro A)62 hsd SB20 λ-

[32]

Vibrio harveyi

BB152, BAA-1119

luxM::Tn5; produces only AI-2

ATCC/[33]

MM77

luxM::Tn5, luxS::CmR; produces neither AI-1 nor AI-2

ATTC/[34]

BB170, BAA-1117

luxN::Tn5; senses only AI-2

ATCC/[33]

Plasmids

pKnock-Km

KmR; ori T; source plasmid for the suicide vectors into Sh. oneidensis

NCCB 3407/[24]

pEX18Ap

AmpR; source plasmid for a replicating plasmid into Sh. oneidensis

Max Schobert, TU Braunschweig/[35]

pPS858

AmpR, GmR; source of the Gentamicin-GFP (Gm-GFP) cassette

Max Schobert, TU Braunschweig/[35]

pRK2013

KmR; helper plasmid for conjugation

DSMZ 5599/[36]

pKnock-luxS

Construction of luxS-ins

KmR; pKnock-Km containing an internal fragment of luxS of Sh. oneidensis

This study

pBA2106

Construction of WTKm

KmR, pKnock-Km containing an upstream fragment of luxS of Sh. oneidensis

This study

pEX18ApGm

For tagging Sh. oneidensis with gfp

KmR, GmR, the Gm-GFP cassette ligated into pEX18Ap

This study