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Table 3 Primers used in this study.

From: The luxS mutation causes loosely-bound biofilms in Shewanella oneidensis

Primer name Primer sequencea Anneal. temp.
CluxS_NotI_for TGG CAG AGA ACT GTT TAG gcggccgc AAC AGG CTC GCT TGA CG 66°C
BluxS_BamHI_rev ATG GCA TAG AGA TCT CCA ggatcc CAG GGC GAT ACA ACG CCA C 66°C
LuxS_EcoRI_beg CAT TAC TTG gaattc TTA CCG TTG ACC ATA CTC 60°C
LuxS_KpnI_mid CAT TGG A ggtacc TC AAT AAT TTC CAC ATC 60°C
  1. aNucleotides in bold indicate the built-in restriction sites.