Table 3 Primers used in this study.
From: The luxS mutation causes loosely-bound biofilms in Shewanella oneidensis
Primer name | Primer sequencea | Anneal. temp. |
|---|---|---|
CluxS_NotI_for | TGG CAG AGA ACT GTT TAG gcggccgc AAC AGG CTC GCT TGA CG | 66°C |
BluxS_BamHI_rev | ATG GCA TAG AGA TCT CCA ggatcc CAG GGC GAT ACA ACG CCA C | 66°C |
LuxS_EcoRI_beg | CAT TAC TTG gaattc TTA CCG TTG ACC ATA CTC | 60°C |
LuxS_KpnI_mid | CAT TGG A ggtacc TC AAT AAT TTC CAC ATC | 60°C |