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Figure 2 | BMC Research Notes

Figure 2

From: Transcript analysis of the extended hyp-operon in the cyanobacteria Nostoc sp. strain PCC 7120 and Nostoc punctiforme ATCC 29133

Figure 2

Transcript levels of the ORFs upstream of the hyp -genes in Nostoc punctiforme ATCC 29133 after nitrogen depletion. Agarose gels showing the amplified PCR products using cDNA prepared from RNA from N. punctiforme cultures 0, 24, 48 and 72 hours after nitrogen depletion The tested genes are the hydrogenase, nitrogenase and ribosome structural genes hupS, nifD and 23S, the hyp-genes hypC and hypF and the ORFs upstream of the hyp-genes (Npun_R0363, Npun_R0364, Npun_R0365, Npun_R0366 and Npun_R03673). All DNA fragments were amplified with PCR using 30 cycles, except for nifD and 23S where 25 and 15 cycles were used, respectively. Negative (-) and positive controls (+) for the PCR reactions are shown.

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