The genes upstream of the hyp -genes can be transcribed together with the hyp -genes in Nostoc punctiforme ATCC 29133. (A) Physical map of the hyp-genes and the ORFs upstream of the hyp-genes in Nostoc punctiforme ATCC 29133. The tsp upstream of hupSL  and the identified tsp upstream of Npun_R0367 are indicated by arrows. The regions amplified by RT-PCR, using the gene specific primers gspNpun_R0364, gspNpun_R0363 and gsphypF, are depicted as arrows under the map and the regions amplified by PCR are depicted as numbered lines under the map. (B) PCR amplification of regions 1-4, 1-5 and 1-6 from cDNA generated with the gene specific primers gspNpun_R0364, gspNpun_R0363 and gsphypF, respectively.