Figure 6

Electrophoretic mobility shift assay of the hupS/ Npun_R0367 promoter region in Nostoc punctiforme ATCC 29133. Electrophoretic mobility shift assay showing specific binding of purified CalA to the N. punctiforme hupS upstream region. C1 - 308 bp control fragment, C2 - 1350 bp control fragment, PhupS - 558 bp hupS immediate upstream region fragment. 100 ng of each fragment and increasing amounts (see label for each lane) of purified histidine-tagged CalA (His-CalA) from Nostoc PCC 7120 were used in the reaction mixtures.