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Table 2 Primer oligonucleotides designed and used to generate or amplify cDNA from cyanobacterial cell cultures after nitrogen depletion

From: Transcript analysis of the extended hyp-operon in the cyanobacteria Nostoc sp. strain PCC 7120 and Nostoc punctiforme ATCC 29133

Primers

Sequence 5'-3'

Product size

N. punctiforme primers

  

hupS forward

ATTATGGCTACAAGGTGGTG

215 bp

hupS reverse

CAACACTGCCTTCAAATACC

 

nifD forward

CCCAATGTGAAGATGAACCT

201 bp

nifD reverse

GCTGATACTTGGCGATAACT

 

rnpb forward

AAGCAATAGCAACCATACAGA

198 bp

rnpb reverse

AATTGATCTGGCGGTATCTT

 

23S forward

GAAACAGCCCAGACCACC

197 bp

23S reverse

AGTGAGCTATTACGCACTC

 

hypC forward

AATCCCCGGACAAATTATAG

128 bp

hypC reverse

ATGTTTCTGCTGCTTCTTGT

 

hypF forward

TTAAAACAGAAATTGTCCCT

216 bp

hypF reverse

CAACATCGTGGTATTCCTTT

 

Npun_0363 forward

TATCATCAAGGACGGCTATT

197 bp

Npun_0363 reverse

TCTCCCCAAAAAGCGATGTC

 

Npun_0364 forward

AACGGTGTGTAGCAGTAGG

179 bp

Npun_0364 reverse

TGGTGGTTTTGGTGGTTTG

 

Npun_0365 forward

GGTTATCTGCCAAGTTATTT

190 bp

Npun_0365 reverse

TTGAAGAGAAACGGCATAAT

 

Npun_0366 forward

TTAACAACGCCAAATCTGAT

220 bp

Npun_0366 reverse

CTAAGAAGATGGCAGGAATT

 

Npun_0367 forward

AGATAGCACAGGGTTTTCAA

219 bp

Npun_0367 reverse

AAATCGGTAATGCCTCTTCA

 

Nostoc PCC 7120 primers

  

hupS forward

TTGTTCAGGCAACACCATGT

154 bp

hupS reverse

GCCTAAGATGCAATCCCAAA

 

23S forward

GCTAAGCGATGTACCGAAGC

199 bp

23S reverse

TAACCCAGAGTGGACGAACC

 

hypC forward

GGAATCCCCGGACAAATTAC

200 bp

hypC reverse

GTTTCGGCTGCTTCTTGTTC

 

hypF forward

GTGTCCCGAATGTGAGGACT

239 bp

hypF reverse

GCGTTGCATCACAAGCTAAA

 

asr0389 forward

ATTTCTGATCAATATGGTCA

202 bp

asr0389 reverse

CCCAGACCCAAAACAATAGC

 

asr0390 forward

CGGCGAGGTTATTTTTGAAG

196 bp

asr0390 reverse

TCTTTGAGAAGAAATGGCATGA

 

alr0391 forward

TTGGCGAGGATAACCGATAG

218 bp

alr0391 reverse

CTGGGGTGGTCAATCAAGTT

 

alr0392 forward

CAAGCTGCTTTGGAAGAGGT

203 bp

alr0392 reverse

ACCGCAATCACCGTCACAAT

 

alr0393 forward

GGGACAGAATGCTAAGGGTA

200 bp

alr0393 reverse

CCCGATTTGGTTCATTCTCC

 

alr0394 forward

CTTTGTTACAGAAGGGTGAA

213 bp

alr0394 reverse

CAGCAGGACTAACTAATAAC

 

RT primers N. punctiforme

  

gsphypF

TCGGATTTTCTTGACGAAGG

-

gspNpun_R0363

TTCGATTATTTCCAGAATCAGC

-

gspNpun_R0364

ATCAGGCACGGTTAAGCATT

-

  1. Primers to amplify cDNA, generated with random primers, from cultures and isolated heterocysts of Nostoc punctiforme ATCC 29133 and cultures of Nostoc sp. strain PCC 7120 are shown as well as gene specific primers for generating cDNA from cultures of Nostoc punctiforme ATCC. Sizes of the resulting PCR product are shown in base pairs (bp). cDNA with gene specific primers were generated through reverse transcriptase reactions (RT).
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BMC Research Notes

ISSN: 1756-0500

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