Table 3 Primer oligonucleotides designed and used to locate novel transcription start points in Nostoc punctiforme ATCC 29133 and to amplify DNA probes for DNA affinity assay and electrophoretic mobility shift assay (EMSA) from N. punctiforme and Nostoc sp. strain PCC 7120 genomic DNA
Primers | Sequence 5'-3' | Product size | Biotinylated |
|---|---|---|---|
5'primer1 0367 | GGGGGTAATCCTCCCAAGTA | - | no |
5'primer2 0367 | TCACCACACATCTCGTAGGC | - | no |
5'primer3 0367 | TGCTGGGGGTCATAACTCTG | - | no |
N. punctiforme F-bio | TTACGCATCTCATCACGGGCCA | yes | |
N. punctiforme R | ACAATACAAAAACACCTAGCCC | 751 bp | no |
N. 7120 F-bio | TGGCTATTAGTTTGTATATTGTT | yes | |
N. 7120 R | TCGCTATTACGTTCCTCTCT | 500 bp | no |
EMSA PhupS F | TTCTAAAATTCTAGGGGGAAATTG | no | |
EMSA PhupS R | GGGCTAGGTGTTTTTGTATTGT | 558 bp | no |
EMSA hupL F | CGCCATTATGAGGAAGCTGT | no | |
EMSA hupL R | CGGTCTTCATCCAACCAATC | 308bp/1350 bp | no |