Figure 4

Results of the multiplex peptide screening approach. NFS-60 were incubated with four controls and eight peptide blocks. Controls included two negative controls without G-CSF and two positive controls with a molarity of 0.1 nM and 3.4 pM G-CSF (adapted control due to increased DMSO concentration). One peptide block consisted of ten peptides in increasing order and was combined with the remaining seven peptide blocks. None of the combinations showed an increase in viability at a total molarity of 10 μM compared to the negative controls. (n = 8 replicates each).