Two-colour RT-MLPA assay. (A) Schematic representation of the RT-MLPA assay design (figure adapted from MRC-Holland, http://www.mlpa.com). (B) Composition of the zebrafish immune response RT-MLPA assay. Expression of genes indicated in blue and red is detected by FAM-labelling and TR-labelling, respectively. In addition, three reference genes (bactin1, ppial, rplp0) are included both in the probe set for FAM-labelling and in the probe set for TR-labelling.