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Table 1 Effect of human milk, haptocorrin free human milk, apo-haptocorrin and holo-haptocorrin on the growth of selected bacterial strains

From: Effect of the vitamin B12-binding protein haptocorrin present in human milk on a panel of commensal and pathogenic bacteria

Species Strain and source [reference] a, b, c, d Human milk Haptocorrin free human milkg Apo-haptocorrinh Holo-haptocorrini
Bifidobacterium breve DSM no 20091 +   - NE
Bifidobacterium infantis DSM no 20088 NE   NE NE
Bifidobacterium bifidum DSM no 20215 +   NE NE
Lactobacillus acidophilus DSM no 20079 +   NE NE
Lactobacillus johnsonii DSM no 10533 +   NE NE
Clostridium difficile DSM no 1296 NE   NE NE
Staphylococcus epidermidis DSM no 20044 +   NE NE
Staphylococcus aureus ATCC 29213 NE   NE NE
Enterococcus faecalis ATCC 29212 NE   NE NE
Enterococcus faecium CCUG 542 T NE   NE NE
Streptococcus thermophilus E2; Tosi et alf - - NE NE
Streptococcus agalactiae CCUG 4208 T NE   NE NE
Escherichia coli ATCC 35218 NE   NE NE
Escherichia coli O157, wild typee NE   NE NE
Escherichia coli O103:H2, wild typee +   NE NE
Escherichia coli O26:H11, wild typee NE   NE NE
Escherichia coli O145:H-, wild typee +   NE NE
Escherichia coli O26:H-, wild typee NE   NE NE
Escherichia coli O126:H20, wild typee NE   NE NE
Escherichia coli O128ab:H-, wild typee +   NE NE
Escherichia coli O128ab:H2 (1), wild typee +   NE NE
Escherichia coli O128ab:H2 (2), wild typee +   NE NE
Escherichia coli O111:H-, wild typee +   NE NE
Escherichia coli O127a:H-; DSM no 8702 +   NE NE
Listeria monocytogenes LO28; - - NE NE
Listeria monocytogenes 4446; Larsen et al, 2002f - - NE NE
Listeria monocytogenes 7291; Larsen et al, 2002f - - NE NE
Salmonella Infantis (1), wild typee NE   NE NE
Salmonella Infantis (2), wild typee NE   NE NE
Salmonella
Enteritidis
(1), wild typee +   NE NE
Salmonella
Enteritidis
(2), wild typee +   NE NE
Salmonella Typhimurium DT104 NE   NE NE
Salmonella Typhimurium DT120 NE   NE NE
Salmonella Typhimurium SL1344 NE   NE NE
  1. Effect of human milk, haptocorrin free human milk, unsaturated human haptocorrin (250 nM), and saturated porcine haptocorrin (250 nM) respectively, assessed by comparing the growth in corresponding well-diffusion assays with growth in well-diffusion assays with added phosphate buffer (negative control) and added erythromycin (positive control). The results represent three independent experiments giving the same result.
  2. + indicates increased growth, e.g. increased density of bacteria around the wells (as compared to buffer control)
  3. -indicates visible inhibition (minimum 5 mm clearance zone)
  4. NE: No effect
  5. a Number in parentheses refers to different isolates.
  6. b All DSM strains were obtained from the German Collection of Microorganisms and Cell Cultures.
  7. c All ATCC strains were obtained from the American Type Culture Collection.
  8. d All CCUG strains were obtained from the Culture Collection, University of Göteborg.
  9. e Isolated by the National Food Institute, Denmark.
  10. f Source of strain.
  11. g Assays with addition of haptocorrin free human milk were only conducted for strains inhibited by human milk.
  12. h Apo-haptocorrin was derived from human milk.
  13. i Holo-haptocorrin was derived from porcine gastric juice.