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Table 1 Effect of human milk, haptocorrin free human milk, apo-haptocorrin and holo-haptocorrin on the growth of selected bacterial strains

From: Effect of the vitamin B12-binding protein haptocorrin present in human milk on a panel of commensal and pathogenic bacteria

Species

Strain and source [reference] a, b, c, d

Human milk

Haptocorrin free human milkg

Apo-haptocorrinh

Holo-haptocorrini

Bifidobacterium breve

DSM no 20091

+

 

-

NE

Bifidobacterium infantis

DSM no 20088

NE

 

NE

NE

Bifidobacterium bifidum

DSM no 20215

+

 

NE

NE

Lactobacillus acidophilus

DSM no 20079

+

 

NE

NE

Lactobacillus johnsonii

DSM no 10533

+

 

NE

NE

Clostridium difficile

DSM no 1296

NE

 

NE

NE

Staphylococcus epidermidis

DSM no 20044

+

 

NE

NE

Staphylococcus aureus

ATCC 29213

NE

 

NE

NE

Enterococcus faecalis

ATCC 29212

NE

 

NE

NE

Enterococcus faecium

CCUG 542 T

NE

 

NE

NE

Streptococcus thermophilus

E2; Tosi et alf

-

-

NE

NE

Streptococcus agalactiae

CCUG 4208 T

NE

 

NE

NE

Escherichia coli

ATCC 35218

NE

 

NE

NE

Escherichia coli

O157, wild typee

NE

 

NE

NE

Escherichia coli

O103:H2, wild typee

+

 

NE

NE

Escherichia coli

O26:H11, wild typee

NE

 

NE

NE

Escherichia coli

O145:H-, wild typee

+

 

NE

NE

Escherichia coli

O26:H-, wild typee

NE

 

NE

NE

Escherichia coli

O126:H20, wild typee

NE

 

NE

NE

Escherichia coli

O128ab:H-, wild typee

+

 

NE

NE

Escherichia coli

O128ab:H2 (1), wild typee

+

 

NE

NE

Escherichia coli

O128ab:H2 (2), wild typee

+

 

NE

NE

Escherichia coli

O111:H-, wild typee

+

 

NE

NE

Escherichia coli

O127a:H-; DSM no 8702

+

 

NE

NE

Listeria monocytogenes

LO28;

-

-

NE

NE

Listeria monocytogenes

4446; Larsen et al, 2002f

-

-

NE

NE

Listeria monocytogenes

7291; Larsen et al, 2002f

-

-

NE

NE

Salmonella Infantis

(1), wild typee

NE

 

NE

NE

Salmonella Infantis

(2), wild typee

NE

 

NE

NE

Salmonella

Enteritidis

(1), wild typee

+

 

NE

NE

Salmonella

Enteritidis

(2), wild typee

+

 

NE

NE

Salmonella Typhimurium

DT104

NE

 

NE

NE

Salmonella Typhimurium

DT120

NE

 

NE

NE

Salmonella Typhimurium

SL1344

NE

 

NE

NE

  1. Effect of human milk, haptocorrin free human milk, unsaturated human haptocorrin (250 nM), and saturated porcine haptocorrin (250 nM) respectively, assessed by comparing the growth in corresponding well-diffusion assays with growth in well-diffusion assays with added phosphate buffer (negative control) and added erythromycin (positive control). The results represent three independent experiments giving the same result.
  2. + indicates increased growth, e.g. increased density of bacteria around the wells (as compared to buffer control)
  3. -indicates visible inhibition (minimum 5 mm clearance zone)
  4. NE: No effect
  5. a Number in parentheses refers to different isolates.
  6. b All DSM strains were obtained from the German Collection of Microorganisms and Cell Cultures.
  7. c All ATCC strains were obtained from the American Type Culture Collection.
  8. d All CCUG strains were obtained from the Culture Collection, University of Göteborg.
  9. e Isolated by the National Food Institute, Denmark.
  10. f Source of strain.
  11. g Assays with addition of haptocorrin free human milk were only conducted for strains inhibited by human milk.
  12. h Apo-haptocorrin was derived from human milk.
  13. i Holo-haptocorrin was derived from porcine gastric juice.