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Table 2 A fragment of the comparison matrix

From: Label-free peptide profiling of Orbitrap™ full mass spectra

Mass MH+

ms2 triggered

ms2 sequenced

msInspect

Peptrix

Progenesis™

Sieve™

 

(for fragmentation)

(proteins found)

Peak intensity (× 103)

Sum peak intensities (× 103)

Peak intensities(× 103)

Peak intensities (× 103)

1741.6671

0

0

0

0

0

0

1741.7007

0

0

473

0

0

652985

1741.7447

1

1

30698

73319

133568

73185

1741.7638

1

0

277640

995497

107051

256383

1741.8094

1

0

3816

222852

14080

0

1741.8397

1

0

41071

146578

58422

78170

1741.8707

1

1

26311

92368

74067

2169410

1741.9122

0

0

3738

19527

6889

0

1741.9355

1

0

202031

95472

564808

273129

1741.9807

0

0

0

0

0

0

1742.008

0

0

1547

0

0

0

1742.0503

0

0

0

0

0

0

1742.0852

0

0

0

0

0

0

1742.12

0

0

0

0

0

0

1742.1472

0

0

0

0

0

101289

1742.193

0

0

0

0

0

45510

1742.2281

0

0

0

2691996

0

0

1742.2594

0

0

0

0

0

0

1742.2909

0

0

1407

69951

0

70092

1742.3208

0

0

0

0

0

3561843

1742.3641

0

0

2474

56134

0

106378

1742.3994

0

0

424

0

0

0

1742.4336

0

0

0

0

0

0

1742.4685

0

0

0

0

0

0

1742.5033

0

0

0

0

0

0

1742.5382

0

0

0

0

0

0

1742.573

0

0

0

0

0

0

1742.6079

0

0

0

0

0

0

1742.6389

0

0

0

0

621012

0

1742.6776

0

0

0

0

0

0

1742.7124

0

0

0

0

0

0

1742.7512

0

0

460

17303

0

60179

1742.7796

0

0

242

5676

0

467398

1742.8157

1

0

1002

67850

82288

43121

1742.8449

0

0

15846

10995

5417

56105

1742.8839

1

1

582

102837

26609

27587

1742.9085

0

0

15849

120678

69898

54135

1742.95

0

0

0

25553

8075

263965

1742.9913

0

0

0

0

0

0

1743.0287

0

0

1005

0

0

0

1743.0563

0

0

0

4248221

0

0

1743.0959

0

0

0

0

0

0

1743.1307

0

0

0

0

0

0

  1. A total of 4 peak-lists containing masses and intensities (1 for each matrix), together with one list of MS2 triggered masses and one list of the MS2 fragmented masses where sequencing succeeded were matched plus or minus 10 ppm with an artificial reference list or grid of masses with fixed distances of 20 ppm. The comparison matrix contains unoccupied space of MH+ mass values, roughly separated by the lines. The triple charged peptide mass 581.69 Da, which when recalculated to the MH+ value of 1743.0515 Da is only detected by Peptrix.