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Figure 2 | BMC Research Notes

Figure 2

From: Antibody recognition of the glycoprotein g of viral haemorrhagic septicemia virus (VHSV) purified in large amounts from insect larvae

Figure 2

Ni-affinity chromatography fractions of protein extracts from Trichoplusia ni insect larvae infected with recombinant baculoviruses, and PAGE of the pooled fractions of G21-465 (insert). Fifty recombinant baculovirus-infected larvae (~10 g) were homogenized in 6 M guanidinimun chloride, 1 M sodium chloride in 40 mM phosphate buffer at pH 7.8 containing 25 mM imidazole. They were then disrupted by sonication and centrifuged until a clear lysate was obtained. A 3-ml Probond (Invitrogen) bed column was used to retain the polyhistidine-tagged recombinant proteins. Bound proteins were eluted using the same buffer with 250 mM imidazole. , protein extract from larvae infected with the G21-465 recombinant baculovirus. ■, protein extract from larvae infected with the G21-507 recombinant baculovirus. *, protein extract from larvae infected with the BacNi baculovirus. Fractions with an absorbance > 0.3 at 280 nm were pooled and electrophoresed in a 4 to 20% polyacrylamide gel. Results of G21-465 stained with Coomassie-blue are shown in the insert.

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