Figure 3

Recognition of solid-phase G21-465 by anti-gpG MAbs and VHSV-hyperimmunized trout sera (hTS). Plates were coated with 1 μg per well of insect-derived G21-465 untreated (- ß) or treated with ß-mercaptoethanol (+ ß), as indicated in the text. Hyperimmunized trout sera (hTS) were obtained by intraperitoneal injections of purified VHSV and diluted 2000-fold. The MAb 2C9 was an anti-VHSV nucleocapsid N-MAb used as a negative control [29]. MAbs were used at 2.5 μg per well. Open bars, ELISA performed with dilution buffer adjusted to pH 6.7. Black bars, ELISA performed with dilution buffer adjusted to pH 7.7. Means and standard deviations from 4 experiments are shown.