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Table 2 Side-by-side comparison between the original and modified Red-mediated gene targeting methods.

From: Reproducible gene targeting in recalcitrant Escherichia coli isolates

  UTI89 APEC1 APEC3E APEC11A APEC16A
Original Red-mediated gene targeting method      
Deletion of type 1 fimbriae cluster 0 0 0 1 0
Deletion of lac operon 2 0 0 0 0
Deletion of lrhA regulator gene 1 0 0 0 0
Modified Red-mediated gene targeting method using 85 bp long homology regions      
Deletion of type 1 fimbriae cluster 2 1 0 0 0
Deletion of lac operon 1 4 0 0 0
Deletion of lrhA regulator gene 1 2 2 1 0
Modified Red-mediated gene targeting method using 500-600 bp long homology regions      
Deletion of type 1 fimbriae cluster 2 0 0 0 0
Deletion of lac operon 18 2 0 5 0
Deletion of lrhA regulator gene 1 1 0 0 0
  1. The number of chloramphenicol resistant and kanamycin sensitive colonies confirmed by PCR is indicated.