Table 2 Side-by-side comparison between the original and modified Red-mediated gene targeting methods.
From: Reproducible gene targeting in recalcitrant Escherichia coli isolates
| Â | UTI89 | APEC1 | APEC3E | APEC11A | APEC16A |
|---|---|---|---|---|---|
Original Red-mediated gene targeting method | Â | Â | Â | Â | Â |
Deletion of type 1 fimbriae cluster | 0 | 0 | 0 | 1 | 0 |
Deletion of lac operon | 2 | 0 | 0 | 0 | 0 |
Deletion of lrhA regulator gene | 1 | 0 | 0 | 0 | 0 |
Modified Red-mediated gene targeting method using 85 bp long homology regions | Â | Â | Â | Â | Â |
Deletion of type 1 fimbriae cluster | 2 | 1 | 0 | 0 | 0 |
Deletion of lac operon | 1 | 4 | 0 | 0 | 0 |
Deletion of lrhA regulator gene | 1 | 2 | 2 | 1 | 0 |
Modified Red-mediated gene targeting method using 500-600 bp long homology regions | Â | Â | Â | Â | Â |
Deletion of type 1 fimbriae cluster | 2 | 0 | 0 | 0 | 0 |
Deletion of lac operon | 18 | 2 | 0 | 5 | 0 |
Deletion of lrhA regulator gene | 1 | 1 | 0 | 0 | 0 |