MicroRNA preparations from individual monogenean Gyrodactylus salaris-a comparison of six commercially available totalRNA extraction kits

Table 1 Summary of the extraction methods and their chemical principles

manufacturer	totalRNA Extraction Kit	lysis	extraction 1		extraction 2
Ambion	mirVana		organic solvents	acid Phenol	solid-phase
Invitrogen	Trizol Plus			Trizol
GeneMole	Molestrips totalRNA basic	Guanidinium-thiocyanat based		beads
Zymo	ZymoReserach RNA miniPrep		solid-phase	column
Amresco	Phenol-free Total RNA Purification				none
Epicentre	MasterPure Complete RNA Purification	SDS based	precipitation, DNAse treatment

Overview of the chemical principles for the assessed totalRNA extraction methods.

ISSN: 1756-0500