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Table 2 totalRNA yield in ng

From: MicroRNA preparations from individual monogenean Gyrodactylus salaris-a comparison of six commercially available totalRNA extraction kits

Number of Gyrodactylus salaris individuals

1

10

100

average per individual

totalRNA Extraction Kit

yield SD

Quality SD

yield SD

quality SD

yield SD

quality SD

yield SD

quality SD

Trizol Plus

2.8 ± 0.8

2.6 ± 1.1

19.0 ± 2.2

9.6 ± 0.4

212.8 ± 50.6

9.9 ± 0.1

2.3 ± 0.4

7.4 ± 4.1

Molestrips totalRNA basic

5.0 ± 0.9

8.3 ± 0.2

75.7 ± 14.2

10.0 ± 0.1

562.7 ± 245.2

9.4 ± 1.3

6.1 ± 1.4

9.2 ± 0.9

mirVana

7.8 ± 0.6

6.7 ± 1.3

91.1 ± 11.6

9.4 ± 0.9

410.9 ± 53.8

9.7 ± 0.5

7.0 ± 2.6

8.6 ± 1.6

ZymoResearch RNA miniprep

20.7 ± 2.4

2.7 ± 0.3

74.0 ± 5.2

3.0 ± 0.1

576.3 ± 124.9

4.0 ± 0.5

11.3 ± 8.2

3.2 ± 0.7

Phenol-free Total RNA Purification

15.4 ± 6.1

9.2 ± 1.2

142.0 ± 27.8

9.9 ± 0.2

1908.5 ± 500.6

9.5 ± 0.9

16.2 ± 2.5

9.5 ± 0.4

MasterPure Complete RNA Purification

25.8 ± 4.3

2.3 ± 0.2

158.4 ± 25.9

3.2 ± 0.5

505.0 ± 59.7

6.2 ± 0.7

15.6 ± 10.4

3.9 ± 2.0

average absolute

12.9 ± 9.2

5.3 ± 3.1

93.4 ± 50.6

7.5 ± 3.4

696.0 ± 608.9

8.1 ± 2.4

9.7 ± 5.6

7.0 ± 2.7

average per individual

12.9 ± 9.2

5.3 ± 3.1

9.3 ± 5.1

7.5 ± 3.4

7.0 ± 6.1

8.1 ± 2.4

  
  1. TotalRNA yield and quality of all assessed totalRNA extraction kits for 1, 10 and 100 Gyrodactylus salaris specimens. RNA-quality is expressed as RQI/RIN values determined by the software following the Experion and 2100 Bioanalyzer electrophoresis systems respectively. The global average over all methods and sample sizes is depicted in bold.
  2. SD-standard deviation