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Table 3 small/miRNA yield in ng

From: MicroRNA preparations from individual monogenean Gyrodactylus salaris-a comparison of six commercially available totalRNA extraction kits

Number of Gyrodactylus salaris individuals 1 10 100 average per individual
totalRNA Extraction Kit smallRNA SD miRNA SD smallRNA SD miRNA SD smallRNA SD miRNA SD smallRNA SD miRNA SD
Trizol Plus 4.3 ± 4.5 1.1 ± 0.9 8.2 ± 11.2 2.5 ± 3.6 22.9 ± 29.6 3.5 ± 5.0 1.8 ± 2.2 0.5 ± 0.6
Molestrips totalRNA basic 4.2 ± 4.7 0.5 ± 0.7 10.8 ± 15.1 2.5 ± 3.5 13.6 ± 8.8 0.9 ± 0.7 1.8 ± 2.1 0.2 ± 0.2
mirVana 1.3 ± n.a. 0.4 ± n.a. 44.4 ± 39.9 6.1 ± 5.6 214.2 ± 81.7 22.4 ± 9.6 2.6 ± 1.6 0.4 ± 0.2
ZymoResearch RNA miniprep 22.3 ± 13.8 9.6 ± 4.8 70.0 ± 28.5 26.6 ± 9.4 333.4 ± 112.0 51.6 ± 16.4 10.9 ± 10.1 4.3 ± 4.7
Phenol-free Total RNA Purification 1.8 ± n.a. 0.2 ± n.a. 56.9 ± 56.6 7.8 ± 7.6 389.3 ± 261.0 33.7 ± 22.0 3.8 ± 2.0 0.4 ± 0.3
MasterPure Complete RNA Purification 19.7 ± 10.0 9.2 ± 3.6 103.4 ± 36.5 25.9 ± 9.0 207.7 ± 47.1 41.1 ± 8.7 10.7 ± 8.8 4.1 ± 4.6
average absolute 8.9 ± 9.5 3.5 ± 4.6 49.0 ± 36.4 11.9 ± 11.3 196.9 ± 154.9 25.6 ± 20.5 5.3 ± 4.3 1.7 ± 2.0
average per individual 9.7 ± 9.2 3.9 ± 4.4 4.9 ± 3.6 1.2 ± 1.1 2.0 ± 1.5 0.3 ± 0.2   
  1. SmallRNA and microRNA yield of all assessed totalRNA extraction kits for 1, 10 and 100 Gyrodactylus salaris specimen. The global average over all methods and sample sizes is depicted in bold.
  2. SD-standard deviation