Table 3 small/miRNA yield in ng
Number of Gyrodactylus salaris individuals | 1 | 10 | 100 | average per individual | ||||
|---|---|---|---|---|---|---|---|---|
totalRNA Extraction Kit | smallRNA SD | miRNA SD | smallRNA SD | miRNA SD | smallRNA SD | miRNA SD | smallRNA SD | miRNA SD |
Trizol Plus | 4.3 ± 4.5 | 1.1 ± 0.9 | 8.2 ± 11.2 | 2.5 ± 3.6 | 22.9 ± 29.6 | 3.5 ± 5.0 | 1.8 ± 2.2 | 0.5 ± 0.6 |
Molestrips totalRNA basic | 4.2 ± 4.7 | 0.5 ± 0.7 | 10.8 ± 15.1 | 2.5 ± 3.5 | 13.6 ± 8.8 | 0.9 ± 0.7 | 1.8 ± 2.1 | 0.2 ± 0.2 |
mirVana | 1.3 ± n.a. | 0.4 ± n.a. | 44.4 ± 39.9 | 6.1 ± 5.6 | 214.2 ± 81.7 | 22.4 ± 9.6 | 2.6 ± 1.6 | 0.4 ± 0.2 |
ZymoResearch RNA miniprep | 22.3 ± 13.8 | 9.6 ± 4.8 | 70.0 ± 28.5 | 26.6 ± 9.4 | 333.4 ± 112.0 | 51.6 ± 16.4 | 10.9 ± 10.1 | 4.3 ± 4.7 |
Phenol-free Total RNA Purification | 1.8 ± n.a. | 0.2 ± n.a. | 56.9 ± 56.6 | 7.8 ± 7.6 | 389.3 ± 261.0 | 33.7 ± 22.0 | 3.8 ± 2.0 | 0.4 ± 0.3 |
MasterPure Complete RNA Purification | 19.7 ± 10.0 | 9.2 ± 3.6 | 103.4 ± 36.5 | 25.9 ± 9.0 | 207.7 ± 47.1 | 41.1 ± 8.7 | 10.7 ± 8.8 | 4.1 ± 4.6 |
average absolute | 8.9 ± 9.5 | 3.5 ± 4.6 | 49.0 ± 36.4 | 11.9 ± 11.3 | 196.9 ± 154.9 | 25.6 ± 20.5 | 5.3 ± 4.3 | 1.7 ± 2.0 |
average per individual | 9.7 ± 9.2 | 3.9 ± 4.4 | 4.9 ± 3.6 | 1.2 ± 1.1 | 2.0 ± 1.5 | 0.3 ± 0.2 | ||