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Table 3 Peritoneal MΦ viability after treatment with immunomodulatory drugsa

From: Effects of immunomodulatory drugs on TNF-α and IL-12 production by purified epidermal langerhans cells and peritoneal macrophages

  Concentration Viability (%)
36 h cultured Mϕ - 95.8 ± 1.5
Prednisone 10-6 M 84.2 ± 2.5
  10-8 M 86.1 ± 1.7
Thalidomide 10-6 M 85.4 ± 3.2
  10-8 M 86.9 ± 2.2
Cyclosporine A 10-6 M 85.5 ± 2.3
  10-8 M 88.2 ± 3.7
Amitriptyline 10-6 M 83.8 ± 2.9
  10-8 M 87.7 ± 0.8
  1. aPurified MΦ were first pre-incubated for 24 h with 10 ng/ml LPS, and then cultured for 36 h in the presence or absence of 10-6 M or 10-8 M prednisone, thalidomide, cyclosporine A, or amitriptyline. Cell viability was then assessed using propidium iodide. All results are shown as mean ± SD for three independent experiments, which did not vary significantly from the control.