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Table 4 In vitro effects of two different concentrations of immunomodulatory drugs on TNF-α, IL-12, and IL-10 production by MΦa

From: Effects of immunomodulatory drugs on TNF-α and IL-12 production by purified epidermal langerhans cells and peritoneal macrophages

  Concentration TNF-α (pg/ml) IL-12 (pg/ml) IL-10 (pg/ml)
36 h cultured Mϕ - 339.3 ± 82.3 255.1 ± 27.5 195.0 ± 12.8
Prednisone 10-6 M 129.6 ± 33.0* 184.6 ± 48.1 209.8 ± 17.3
  10-8 M 138.2 ± 6.0* 149.4 ± 7.5** 193.5 ± 29.8
Thalidomide 10-6 M 100.9 ± 9.0** 149.0 ± 14.9* 221.2 ± 22.7
  10-8 M 228.6 ± 78.9 192.9 ± 63.1 160.1 ± 29.8
Cyclosporine A 10-6 M 117.4 ± 59.7* 190.6 ± 10.8 212.4 ± 4.8
  10-8 M 215.4 ± 32.3 150.1 ± 11.9** 184.2 ± 21.9
Amitriptyline 10-6 M 139.7 ± 49.2* 157.0 ± 27.6* 200.2 ± 10.2
  10-8 M 198.8 ± 47.2 147.1 ± 47.4* 207.5 ± 8.1
  1. aPurified MΦ were pre-incubated for 24 h with 10 ng/ml LPS and cultured for 36 h in the presence or absence of 10-6 M or 10-8 M prednisone, thalidomide, cyclosporine A, or amitriptyline. TNF-α, IL-12, and IL-10 levels in the culture supernatant were quantified by ELISA. All results are shown as mean ± SD for three independent experiments. *p < 0.05 vs. control. **p < 0.01 vs. control.