Effects of immunomodulatory drugs on TNF-α and IL-12 production by purified epidermal langerhans cells and peritoneal macrophages

Table 4 In vitro effects of two different concentrations of immunomodulatory drugs on TNF-α, IL-12, and IL-10 production by MΦ^a

	Concentration	TNF-α (pg/ml)	IL-12 (pg/ml)	IL-10 (pg/ml)
36 h cultured Mϕ	-	339.3 ± 82.3	255.1 ± 27.5	195.0 ± 12.8
Prednisone	10^-6 M	129.6 ± 33.0*	184.6 ± 48.1	209.8 ± 17.3
	10^-8 M	138.2 ± 6.0*	149.4 ± 7.5**	193.5 ± 29.8
Thalidomide	10^-6 M	100.9 ± 9.0**	149.0 ± 14.9*	221.2 ± 22.7
	10^-8 M	228.6 ± 78.9	192.9 ± 63.1	160.1 ± 29.8
Cyclosporine A	10^-6 M	117.4 ± 59.7*	190.6 ± 10.8	212.4 ± 4.8
	10^-8 M	215.4 ± 32.3	150.1 ± 11.9**	184.2 ± 21.9
Amitriptyline	10^-6 M	139.7 ± 49.2*	157.0 ± 27.6*	200.2 ± 10.2
	10^-8 M	198.8 ± 47.2	147.1 ± 47.4*	207.5 ± 8.1

^aPurified MΦ were pre-incubated for 24 h with 10 ng/ml LPS and cultured for 36 h in the presence or absence of 10^-6 M or 10^-8 M prednisone, thalidomide, cyclosporine A, or amitriptyline. TNF-α, IL-12, and IL-10 levels in the culture supernatant were quantified by ELISA. All results are shown as mean ± SD for three independent experiments. *p < 0.05 vs. control. **p < 0.01 vs. control.

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