A rapid genotyping method for an obligate fungal pathogen, Puccinia striiformis f.sp. tritici, based on DNA extraction from infected leaf and Multiplex PCR genotyping

Table 1 Description of three PCR multiplexes enabling the genotyping of 20 Puccinia striiformis f.sp. tritici SSR

Multiplex	SSR Locus	Florescence	Allele size range	Reference
Multiplex 1	RJN5	Black	225-231	[13]
	RJN6	Black	310-322	[13]
	RJN13	Green	150-153	[13]
	RJN3	Green	339-347	[13]
	RJO21	Blue	167-182	[16]
	RJN4	Blue	258-264	[13]
	RJN8	Blue	306-318	[13]
	RJO18	Blue	334-360	[16]
Multiplex 2	RJN12	Black	192-200	[13]
	RJN10	Black	225-231	[13]
	RJO24	Black	273-308	[16]
	RJO4	Green	201-207	[16]
	RJO20	Green	282-293	[16]
	RJN11	Blue	173-185	[13]
	RJN9	Blue	335-337	[13]
Multiplex 3	RJN2	Black	172-196	[13]
	WU6	Black	211-213	Provided by X. Chen
	RJO3	Green	202-204	[16]
	WU12	Green	325-334	Provided by X. Chen
	RJO27	Blue	217-243	[16]

ISSN: 1756-0500