Monomeric alpha-amylase inhibitors expressed in Butte 86. In Clustal W2 sequence alignments in Figs. 1, 2, 3, 4 and 5, identical residues are denoted with asterisks, conserved substitutions with colons and semi-conserved substitutions with periods and predicted signal peptide cleavage sites are indicated by the arrow. The N-terminus of a WMAI determined by Kashlan and Richardson  is shown by a bracket. Unique peptides that distinguish WMAI Bu-1 from WMAI Bu-2 are enclosed in shaded boxes. Total MS/MS coverages of WMAI Bu-1 in protein spots 289 and 528 from Dupont et al.  were 61 and 64%, respectively (Additional file 5).