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Table 1 Identification of Butte 86 contigs for alpha-amylase/protease inhibitors, similarity to sequences in NCBI and characteristics of encoded proteins

From: The spectrum of low molecular weight alpha-amylase/protease inhibitor genes expressed in the US bread wheat cultivar Butte 86

Butte 86 Contig Name

# ESTs

Representative EST

Coding region bp

Closest match from T. aestivum in NCBI nr1

Identity

Cultivar

MW2

#aa2

pI2

WMAI Bu-1

11

[Genbank:BQ804583]

456

[Genbank:AK336166]

456/456

Chinese Spring

13155

121

6.19

WMAI Bu-2

3

[Genbank:BQ805680]

456

[Genbank:AK336166]3

440/456

Chinese Spring

13056

121

5.37

WDAI Bu-1

5

[Genbank:BQ805792]

465

[Genbank:AK330823]4

438/465

Chinese Spring

13337

124

6.66

WDAI Bu-2

2

[Genbank:BQ805854]

465

[Genbank:DQ019829]

426/426

Chinese Spring

13337

124

6.66

WDAI Bu-3

2

[Genbank:BQ805829]

465

[Genbank:AK330823]

464/465

Chinese Spring

13181

124

6.49

WDAI Bu-4

1

[Genbank:BQ806690]

459

[Genbank:DQ019827]5

425/426

Chinese Spring

13191

124

5.23

WTAI-CM1 Bu-1

8

[Genbank:BQ804568]

438

[Genbank:X17575]

437/438

Chinese Spring

13095

120

6.72

WTAI-CM2 Bu-1

8

[Genbank:BQ804416]

438

[Genbank:X17575]6

415/440

Chinese Spring

13034

120

6.23

WTAI-CM3 Bu-1

20

[Genbank:BQ804159]

507

[Genbank:AK330649]

507/507

Chinese Spring

15832

143

6.66

WTAI-CM3 Bu-2

7

[Genbank:BQ804161]

507

[Genbank:AK330649]

484/507

Chinese Spring

15916

143

6.66

WTAI-CM16 Bu-1

10

[Genbank:BQ804374]

432

[Genbank:X17573]

432/432

Chinese Spring

13437

119

5.02

WTAI-CM17 Bu-1

5

[Genbank:BQ804549]

432

[Genbank:X59791]

431/431

Timaglen

13502

120

4.87

WASI Bu-1

2

[Genbank:BQ806745]

5437

[Genbank:AK334580]

529/546

Chinese Spring

19633

180

6.77

WASI Bu-2

3

[Genbank:BQ806454]

6128

[Genbank:AK334580]

611/612

Chinese Spring

19690

181

6.77

CMx Bu-1

2

[Genbank:BQ805931]

438

[Genbank:X75608]

397/425

Chinese Spring

14009

122

6.14

CMx Bu-2

4

[Genbank:BQ806494]

441

[Genbank:X75608]

426/441

Chinese Spring

14027

122

8.08

CMx Bu-3

1

[Genbank:BQ804611]

435

[Genbank:X75608]

395/422

Chinese Spring

13891

121

8.02

WTI Bu-1

1

[Genbank:BQ806495]

4179

nd10

na11

na11

13290

122

5.47

WCI Bu-1

2

[Genbank:BQ805289]

432

[Genbank:AJ422078]

347/348

San Pastore

12962

119

7.42

  1. 1 Determined by BLASTn of NCBI nr database limited to T. aestivum using coding region only, database last searched on 4/1/11.
  2. 2 Mature protein encoded by contig after signal peptide cleavage at site predicted by Signal P [20]. Assumes additional N-terminal processing for WMAI and WDAI.
  3. 3 Coding region is a perfect match with [Genbank:FJ874627] from T. turgidum subsp. dicoccoides isolate 33-13A that was added to NCBI after database used for MS/MS analysis in Dupont et al. [17] was created.
  4. 4 425/426 bp of coding region matches [Genbank:FJ897099] from Aegilops tauschii PI499264-2(D) that was added to NCBI after database used for MS/MS analysis in Dupont et al. [17] was created.
  5. 5 Coding region is a perfect match with [Genbank:FJ897030] from T. turgidum subsp. dicoccoides isolate TD30-49-18 that was added to NCBI after database used for MS/MS analysis in Dupont et al. [17] was created.
  6. 6 Coding region is a perfect match with [Genbank:X55454] from T. turgidum subsp. durum cv Desp. GATH.
  7. 7 Does not include coding sequences for signal peptide.
  8. 8 Assumes that C at position 537 of BQ806454 is deleted.
  9. 9 Does not include sequences encoding the first portion of the signal peptide.
  10. 10 nd, no significant similarity found to any Triticum aestivum sequences.
  11. 11 na, not applicable.
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BMC Research Notes

ISSN: 1756-0500

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