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Table 1 Identification of Butte 86 contigs for alpha-amylase/protease inhibitors, similarity to sequences in NCBI and characteristics of encoded proteins

From: The spectrum of low molecular weight alpha-amylase/protease inhibitor genes expressed in the US bread wheat cultivar Butte 86

Butte 86 Contig Name # ESTs Representative EST Coding region bp Closest match from T. aestivum in NCBI nr1 Identity Cultivar MW2 #aa2 pI2
WMAI Bu-1 11 [Genbank:BQ804583] 456 [Genbank:AK336166] 456/456 Chinese Spring 13155 121 6.19
WMAI Bu-2 3 [Genbank:BQ805680] 456 [Genbank:AK336166]3 440/456 Chinese Spring 13056 121 5.37
WDAI Bu-1 5 [Genbank:BQ805792] 465 [Genbank:AK330823]4 438/465 Chinese Spring 13337 124 6.66
WDAI Bu-2 2 [Genbank:BQ805854] 465 [Genbank:DQ019829] 426/426 Chinese Spring 13337 124 6.66
WDAI Bu-3 2 [Genbank:BQ805829] 465 [Genbank:AK330823] 464/465 Chinese Spring 13181 124 6.49
WDAI Bu-4 1 [Genbank:BQ806690] 459 [Genbank:DQ019827]5 425/426 Chinese Spring 13191 124 5.23
WTAI-CM1 Bu-1 8 [Genbank:BQ804568] 438 [Genbank:X17575] 437/438 Chinese Spring 13095 120 6.72
WTAI-CM2 Bu-1 8 [Genbank:BQ804416] 438 [Genbank:X17575]6 415/440 Chinese Spring 13034 120 6.23
WTAI-CM3 Bu-1 20 [Genbank:BQ804159] 507 [Genbank:AK330649] 507/507 Chinese Spring 15832 143 6.66
WTAI-CM3 Bu-2 7 [Genbank:BQ804161] 507 [Genbank:AK330649] 484/507 Chinese Spring 15916 143 6.66
WTAI-CM16 Bu-1 10 [Genbank:BQ804374] 432 [Genbank:X17573] 432/432 Chinese Spring 13437 119 5.02
WTAI-CM17 Bu-1 5 [Genbank:BQ804549] 432 [Genbank:X59791] 431/431 Timaglen 13502 120 4.87
WASI Bu-1 2 [Genbank:BQ806745] 5437 [Genbank:AK334580] 529/546 Chinese Spring 19633 180 6.77
WASI Bu-2 3 [Genbank:BQ806454] 6128 [Genbank:AK334580] 611/612 Chinese Spring 19690 181 6.77
CMx Bu-1 2 [Genbank:BQ805931] 438 [Genbank:X75608] 397/425 Chinese Spring 14009 122 6.14
CMx Bu-2 4 [Genbank:BQ806494] 441 [Genbank:X75608] 426/441 Chinese Spring 14027 122 8.08
CMx Bu-3 1 [Genbank:BQ804611] 435 [Genbank:X75608] 395/422 Chinese Spring 13891 121 8.02
WTI Bu-1 1 [Genbank:BQ806495] 4179 nd10 na11 na11 13290 122 5.47
WCI Bu-1 2 [Genbank:BQ805289] 432 [Genbank:AJ422078] 347/348 San Pastore 12962 119 7.42
  1. 1 Determined by BLASTn of NCBI nr database limited to T. aestivum using coding region only, database last searched on 4/1/11.
  2. 2 Mature protein encoded by contig after signal peptide cleavage at site predicted by Signal P [20]. Assumes additional N-terminal processing for WMAI and WDAI.
  3. 3 Coding region is a perfect match with [Genbank:FJ874627] from T. turgidum subsp. dicoccoides isolate 33-13A that was added to NCBI after database used for MS/MS analysis in Dupont et al. [17] was created.
  4. 4 425/426 bp of coding region matches [Genbank:FJ897099] from Aegilops tauschii PI499264-2(D) that was added to NCBI after database used for MS/MS analysis in Dupont et al. [17] was created.
  5. 5 Coding region is a perfect match with [Genbank:FJ897030] from T. turgidum subsp. dicoccoides isolate TD30-49-18 that was added to NCBI after database used for MS/MS analysis in Dupont et al. [17] was created.
  6. 6 Coding region is a perfect match with [Genbank:X55454] from T. turgidum subsp. durum cv Desp. GATH.
  7. 7 Does not include coding sequences for signal peptide.
  8. 8 Assumes that C at position 537 of BQ806454 is deleted.
  9. 9 Does not include sequences encoding the first portion of the signal peptide.
  10. 10 nd, no significant similarity found to any Triticum aestivum sequences.
  11. 11 na, not applicable.