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Table 2 Association of Butte 86 contigs with alpha-amylase/protease inhibitors identified by MS/MS in a previous proteomic analysis of Butte 86 flour

From: The spectrum of low molecular weight alpha-amylase/protease inhibitor genes expressed in the US bread wheat cultivar Butte 86

Butte 86 Contig # Peptides1 # Unique Peptides2 Spot Number3 MS/MS ID4
WMAI Bu-1 11 2 289*, 528* [PRF:223520]5
WMAI Bu-2 9 0 nd na
WDAI Bu-1/WDAI Bu-26 9 2 280, 283, 285, 312*, 313 [SwissProt:P01085]5
WDAI Bu-3 9 3 281, 283* TC11_338524
WDAI Bu-4 12 3 283, 286* [Genbank:AAV91972]
WTAI-CM1 Bu-1 6 3 312, 313* TC11_340510
WTAI-CM2 Bu-1 9 5 280*, 285* [SwissProt:P16851]
WTAI-CM3 Bu-1 16 6 264*, 265*, 272 [SwissProt:P17314]
WTAI-CM3 Bu-2 14 4 264, 265 RS_UWI_15430
WTAI-CM16 Bu-1 17 12 266, 282, 284*, 286* [SwissProt:P16159]
WTAI-CM17 Bu-1 11 6 274*, 282* [Genbank:CAA42453]
WASI Bu-17 12 1 244* [SwissProt:P16347]5
WASI Bu-2 11 0 nd na
CMx Bu-1 8 2 280, 285, 313 TC11_320696
CMx Bu-2 12 1 281, 286, 290* TC11_308146
CMx Bu-3 14 2 281*, 290 TC11_309398
WTI Bu-17 2 2 2868, 278 TC11_315743
WCI Bu-1 6 6 277*, 278* [Genbank:CAD19440]
  1. 1 Number of distinct peptides from Dupont et al. [17] that could be assigned to the protein encoded by Butte 86 contig.
  2. 2 Number of peptides from Dupont et al. [17] that could be assigned exclusively to the protein encoded by Butte 86 contig.
  3. 3 Spot numbers from 2-DE analysis of Dupont et al. [17]. * indicates that the sequence was the predominant protein identified in the spot. nd indicates that unique peptides for the protein encoded by the contig were not detected in any spots.
  4. 4 MS/MS ID reported in Dupont et al. [17]. Accession numbers from DFCI Wheat Gene Index Release 11.0 are denoted with the prefix TC11_ and from HarvEST 1.14 are denoted with RS_UWI_. na indicates not applicable.
  5. 5 Protein sequence determined by Edman degradation.
  6. 6 Mature proteins encoded by WDAI Bu-1 and WDAI Bu-2 are identical.
  7. 7 Missing all or part of coding region for signal sequence.
  8. 8 Spot may contain an additional form of WTI not represented in Butte 86 EST collection.