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Figure 3 | BMC Research Notes

Figure 3

From: An improved PCR strategy for fast screening of specific and random integrations in rAAV-mediated gene targeted cell clones

Figure 3

The screening PCRs can semi-quantitatively detect the proportions of KO and RI DNA. Semi-quantitative screening PCRs (BRCA1-KO-PCR (1952 bp) and 5'-AAV-RI-PCR (1943 bp)) on genomic DNA (50 ng) mixed from genomic DNA from BRCA1 knockout (KO) and random integration (RI) clones in a ratio of 100%, 90%, 80%, 50%, 20%, 10%, and 0% (KO: RI), respectively. NC, negative control. KO %, percentage of gene KO; RI %, percentage of random integration.

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BMC Research Notes

ISSN: 1756-0500

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