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Figure 4 | BMC Research Notes

Figure 4

From: An improved PCR strategy for fast screening of specific and random integrations in rAAV-mediated gene targeted cell clones

Figure 4

Screening PCRs for BRCA1 KO in rAAV-mediated BRCA1 KO cells. Screening PCRs (Neo-PCR (490 bp), BRCA1-KO-PCR (1952 bp), 5'-AAV-RI-PCR (1943 bp) and 3'-AAV-RI-PCR (1728 bp)) were performed using one μl cell lysate (A). Genotype summary of clones that are BRCA1 KO only (), BRCA1 KO with random integration (#), random integration without the ITR sequences (*), and random integration with the ITR sequences (the rest) are listed in (B). "a", positive control (DNA, which is positive for the four screening PCRs); "b" negative control.

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