Figure 2

Conservation of proline and phenylalanine residues in all DGATs. Multiple sequence alignment was performed using the ClustalW algorithm and 117 DGAT protein sequences from 70 organisms (listed in Table 1). DGAT sequence name is on the left of alignment followed by the start of the amino acid residue of each DGAT protein sequence. The completely conserved proline and phenylalanine residues are highlighted in red on yellow. Other color code and related information are described in "Methods" section. (A) Motif 1 (P Block): the completely conserved proline residue in all DGATs, (B) Motif 2 (FG Block): the completely conserved tryptophan residue followed by a highly conserved glycine residue in all DGATs, (C) Motif 3 (P-1 Block): the almost completely conserved proline residue except one in all DGATs.